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1994-10-25
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Document 2650
DOCN M94A2650
TI HIV-specific IgG3 for early detection of uninfected children born to
HIV-infected mothers.
DT 9412
AU Caselli D; Maccabruni A; Marconi M; Pasinetti G; Stronati M; Bossi G;
Arico M; Dept. of Pediatrics, University of Pavia, IRCCS Policlinico
San; Matteo & Neonatology, Osp. Riuniti, Bergamo, Italy.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):256 (abstract no. PB0455). Unique
Identifier : AIDSLINE ICA10/94369925
AB BACKGROUND: we still lack a simple, low cost test for early
identification of uninfected children born to HIV seropositive mothers.
We previously reported a novel method to detect HIV infected children
using HIV-specific IgG3 antibodies (aHIV-G3). Now we report the result
of a prospective study of aHIV-G3 in newborns of seropositive mothers
and their concordance with infection status. PATIENTS AND METHODS: 31
consecutive children born to HIV-positive mothers were enrolled. All of
them were first tested during the first 15 days of life and then
retested by the age of 3 months. Results were compared with infection
status defined as serum negativization or confirmed diagnosis of HIV
infection assessed by conventional serological tests, PCR and virus
isolation. aHIV-G3 assay was performed using a commercial kit (Bio Rad,
Richmond CA) modified with overnight incubation with serum and FBS and
than matched with IgG specific antibodies. Anti-mouse IgG MoAb alkaline
phosphatase coniugated was finally used. RESULTS: Of the 18/31 (58%) pts
who were aHIV-G3 negative at birth, 16 remained negative and are
non-infected, 1 became aHIV-G3 positive at 3 months and is infected, and
1 died at 3 months of progressive disease with total IgG3 levels < 3rd
percentile. Of the 13/31 (42%) pts who tested positive at birth, 5
remained G3 positive troughout the study period and were documented to
be infected, while the 8 who seroreverted cleared aHIV-G3 at a median
age of 2 months (range 1-8 months). CONCLUSION: This prospective study
confirmed that aHIV-G3 is a novel, simple, low-technology, low cost
assay that, when performed at birth and at 3 months, predicted correct
infection status in 28/31 pts (90%). Late (3 to 8 months) aHIV-G3
clearance and AIDS-dependent IgG3 depletion were possible pitfalls.
DE *AIDS Serodiagnosis Female Follow-Up Studies Human HIV
Antibodies/*BLOOD HIV Infections/*DIAGNOSIS/IMMUNOLOGY/TRANSMISSION
HIV Seronegativity/*IMMUNOLOGY IgG/*BLOOD Infant Infant, Newborn
Italy Predictive Value of Tests Pregnancy MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).